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KMID : 0377519840090020177
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Chung-Ang Journal of Medicine
1984 Volume.9 No. 2 p.177 ~ p.186
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Relationships between Becterial Growth, Yield of Tuberculoprotein and Biological Potency of Tuberculin
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Choi Chul-Soon
Kwak Byong-Eun
Yang Yong-Tae
Chung Sang-In
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Abstract
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In the epidemiological survey of tuberculosis with tuberculin antigen, there are two problems. The one is a lack sensitivity or occurrence of false negative reaction and the other is a low specificity or the development of false positive reaction. During the last three decades, the general objective of research works on tuberculin preparations was to obtain a more purified tuberculin that provides a higher sensitivity and specificity. However, there have been little emphasis on the selection of media which yield the maximum quantities of active tuberculoprotein. The purpose of present work was to compare the amount of the bacterial growth, yield of tuberculoprotein and biological potency of tuberculins prepared from four different synthetic media. In addition, sensitivity and specificity of heart-concentrated synthetic medium(HCSM), of purified protein dervative (PPD) and of Seibert’s Fraction A(SFA) were tested in guinea pigs sensitized with M. tuberculosis-bovis complex and atypical mycobacteria. The results are summarized as follows: 1. The largest amount of bacterial growth of M. bovis occurred in Dorset-Henley’s medium at 7 weeks cultivation and it was 1.7 times or above compared with those of Lind’s medium, Long-Seibert’s medium and Sauton’s medium. 2. The yield of tuberculoprotein in the culture medium was the highest in Lind’s medium 13 weeks cultivation and was 1.5 times as much compared with that of Dorset Henley’s medium, 1.7 times of Long-Seibert’s medium and 7.4 times of Sauton’s medium, respectively. 3. The highest biological titre, 1:400, of tuberculin appeared in the HCSM tuberculin derived from either Sauton’s medium or Long-Seibert’s medium. The biological titres of tuberculins prepared from Dorset-Henley’s medium and Long-Seibert’s medium were 1:300 and 1:200, respectively. 4. With the HCSM tuberculin, there observed little differences in the sensitivity and specificity between PPD tuberculin and SFA tuberculin, but high degree of cross reactions were elicited with HCSM tuberculin. These results indicated that there could exist none of simple relationships between bacterial growth, tuberculin yield and biological potency of tuberculin, and therefore, culture media should be carefully selected for production of tuberculin.
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KEYWORD
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